This is due to the freezing technique and because the semen is stored at such a low temperature (-196 C). Samples stored more than 20 years have been used to create successful pregnancies and healthy babies. Appointments to cryopreserve semen are scheduled Monday through Friday from 7:30 a. We recommend that you do not have sex for two to three days before each appointment. For best results, you should come into the clinic and collect your sample.